Dopamine

Dopamine: methods and protocols

by Kabbani, Nadine

Type Book
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Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery. In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this colle…

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Title Statements

  • Full title :Dopamine : methods and protocols / edited by Nadine Kabbani.

Summary

Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery. In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson's disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.

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Edition Statements

  • Publication Date:©2013.

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York
  • Language:eng
  • Publication Date:©2013.

The Physical Object

  • Description:1 online resource (xi, 312 pages) : : illustrations (some color)

Edition Identifiers

  • ISBN:9781627032513, 1627032517, 1627032509, 9781627032506
  • DOI:10.1007/978-1-62703-251-3
  • oclc:ocn824946048
  • LC Classification:QP563.D66
  • Dewey Decimal:612.8/042

Table of Contents

Detection of Cell Surface Dopamine Receptors -- Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells -- Methods of Dopamine Research in Retina Cells -- Capture of D2 Dopamine Receptor Signaling Complexes in Striatal Cells for Mass Spectrometry Proteomic Analysis -- Modeling Spatial Aspects of Intracellular Dopamine Signaling -- A Biophysical Approach for the Study of Dopamine Receptor Oligomerization -- Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET (SRET) Technology -- BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling -- Dopaminergic Regulation of Dendritic Calcium: Fast Multi-Site Calcium Imaging -- Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosolic End of Transmembrane Region 6 -- A Molecular Genetic Approach to Uncovering the Differential Functions of Dopamine D2 Receptor Isoforms -- Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish -- Application of Cell-Specific Isolation to the Study of Dopamine Signaling in Drosophila -- Regulation of Do…

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Subjects

Biomedicine biomedische wetenschappen Dopamine Dopamine -- Laboratory manuals Dopamine -- Manuels de laboratoire Geneeskunde (algemeen) Laboratory Manual Laboratory manuals Manuels de laboratoire Medicine Medicine (General) Neurobiologie Neurobiology Neurochemie Neurochemistry Neuroscience Neurosciences neurowetenschap

Writers

Kabbani, Nadine

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