Enhancer RNAs: methods and protocols

by Ørom, Ulf Andesson

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Title Statements

Full title :Enhancer RNAs : methods and protocols / edited by Ulf Andesson Ørom, Max Planck Institute for Molecular Genetics, Berlin, Germany.

Summary

"This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Enhancer RNAs: Methods and Protocols aims to ensure successful results in this rapidly developing field."--OCLC.

Edition Statements

Publication Date:2017

Publication Statements

Publisher(s):Humana Press, Springer
Place of Publication:New York
Language:eng
Publication Date:2017

The Physical Object

Description:1 online resource (1 recurs en línia (xi, 252 pàgines) :) : : il·lustracions (algunes en color).

Edition Identifiers

ISBN:9781493940356, 149394035X
oclc:ocn986630844

Conté: Cellular fractionation and isolation of chromatin-associated RNA -- Knockdown of nuclear-located enhancer RNAs and long ncRNAs using locked nucleic acid GapmeRs -- Visualization of enhancer-derived noncoding RNA -- UV-RNA immunoprecipitation (UV-RIP) protocol in neurons -- Mapping long noncoding RNA chromatin occupancy using capture hybridization analysis of RNA targets (CHART) -- Detecting long-range enhancer-promoter interactions by quantitative chromosome conformation capture -- Deciphering noncoding RNA and chromatin interactions : multiplex chromatin interaction analysis by paired-end tag sequencing (mChIA-PET) -- Identification of transcribed enhancers by genome-wide chromatin imunoprecipitation sequencing -- Global run-on sequencing (GRO-Seq) -- Computational approaches for mining GRO-Seq data to identify and characterize active enhancers -- Evaluating the stability of mRNAs and noncoding RNAs -- Novel method to quantify RNA-protein interactions in situ using FMTRIP and proximity ligation -- In silico promoter recognition from deepCAGE data -- Bioinformatics pipeline for transcriptome sequencing analysis -- CRISPR/Cas9 genome editing in embryonic stem cells -- target…