Enzyme-mediated ligation methods

by Nuijens, Timo Schmidt, Marcel

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Title Statements

Full title :Enzyme-mediated ligation methods / edited by Timo Nuijens, Marcel Schmidt.

Summary

"This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls." -- Prové de l'editor.

Edition Statements

Publication Date:2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

Notes

Conté: Preface...Table of Contents...Contributing Authors... 1. Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug ConjugatesRémy Gébleux, Manfred Briendl, Ulf Grawunder, and Roger R. Beerli 2. Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL)Hejia Henry Wang and Andrew Tsourkas 3. Cyclizing Disulfide-Rich Peptides using Sortase AAkello J. Agwa, David J. Craik, and Christina I. Schroeder 4. Chemo-Enzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1Marcel Schmidt and Timo Nuijens 5. Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE)Igor Rupniewski and David Rabuka 6. Butelase 1-Mediated Ligation of Peptides and ProteinsXinya Hemu, Xiaohong Zhang, Xiaobao Bi, Chuan-Fa Liu, and James P. Tam 7. Trypsiligase-Catalyzed Peptide and Protein LigationSandra Liebscher and Frank Bordusa 8. Site-Specific Antibody-Drug Conjugation Using Microbial TransglutaminaseStephan Dickgiesser, Lukas Deweid, Roland Kellner, Harald Kolmar, and Nicolas Rasche 9. Tailoring Activity and Selectivity of Microbial Transglutaminase Lukas Deweid, Olga Avrutina, and Harald Kolmar 10. SpyLigase-Catalyzed Modification of AntibodiesVanessa Siegmund, Birgit Piater, Frank Fischer, and Harald Kolmar 11. Peptide Cyclization Catalyzed by Cyanobactin MacrocyclasesWael E. Houssen 12. In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinisKaren S. Harris, Simon Poon, Pedro Quimbar, and Marilyn A. Anderson 13. Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed LigationJan Grünewald, Ansgar Brock, and Bernhard H. Geierstanger 14. Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and ImmobilizationJoseph G. Plaks and Joel L. Kaar 15. BioID as a Tool for Protein Proximity Labeling in Living CellsRhiannon M. Sears, Danielle G. May, and Kyle J. Roux 16. N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live CellsSamuel H. Ho and David A. Tirrell 17. Tubulin Tyrosine Ligase-Mediated Modification of ProteinsMarcus Gerlach, Tina Stoschek, Heinrich Leonhardt, Christian P.R. Hackenberger, Dominik Schumacher, and Jonas Helma 18. Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of TyrosineJorick J. Bruins, Criss van de Wouw, Jordi F. Keijzer, Bauke Albada, and Floria L. van Delft.