High-throughput metabolomics: methods and protocols
"This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data spa…
Read More ↓Title Statements
- Full title :High-throughput metabolomics : methods and protocols / edited by Angelo D'Alessandro.
Summary
"This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine." - Prové de l'editor.
Read More ↓Edition Statements
- Publication Date:2019
- Edition:1st ed. 2019
Publication Statements
- Publisher(s):Imprint: Humana, Springer New York
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2019
The Physical Object
- Description:1 recurs en línia (XV, 459 pàgines) : : 83 il·lustracions, 63 il·lustracions en color
Edition Identifiers
- ISBN:9781493992362, 1493992368
- DOI:10.1007/978-1-4939-9236-2
- oclc:on1139503362
Table of Contents
Conté: Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells -- High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods -- High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research -- Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications -- CE-MS for Metabolomics: A Comparison with Other Techniques -- Overview of Lipid Mass Spectrometry and Lipidomics -- LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins -- Untargeted and Semi-Targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics -- HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle -- Quantification of D- and L-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry -- Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples -- Untargeted Metabolomics of Plant Leaf Tissues -- Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling -- Quantifying Intermediary Metabolism and Lipogene…
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