High-throughput RNAi screening: methods and protocols

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High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries.

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Title Statements

Full title :High-throughput RNAi screening : methods and protocols / editors David O. Azorsa, Institute of Molecular Medicine, Phoenix Children's Hospital, Phoenix, Arizona, USA ; Shilpi Arora, Constellation Pharmaceuticals, Cambridge, Massachusetts, USA.

Summary

Edition Statements

Publication Date:2016

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2016

The Physical Object

Description:1 online resource (xi, 262 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9781493963379, 1493963376
oclc:ocn957740693
LC Classification:QP623.5.S63
Dewey Decimal:572.8/8

Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening -- Optimization of Transfection Conditions for siRNA Screening -- High Throughput siRNA Screening Using Reverse Transfection -- Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells -- Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform -- A Protocol for a High-Throughput Multiplex Cell Viability Assay -- RNAi Screening of Leukemia Cells Using Electroporation -- Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening -- In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity -- Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens -- In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development -- High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans -- Design and Methods of Large-Scale RNA Interference Screens in Drosophila -- Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity -- RNAi-Assisted Genome Evolution (…

Subjects

Criblage à haut débit (Développement de… Drug Discovery Drug Evaluation, Preclinical -- methods High throughput screening (Drug develop… High throughput screening (Drug develop… Interférence ARN Interférence ARN -- Manuels de laborato… Laboratory Manual Laboratory manuals Manuels de laboratoire Medical genetics RNA Interference RNA interference -- Laboratory manuals