Membrane proteins: engineering, purification and crystallization

by Shukla, Arun Kumar

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Title Statements

Full title :Membrane proteins : engineering, purification and crystallization / edited by Arun K. Shukla.

Edition Statements

Publication Date:©2014
Edition:First edition

Publication Statements

Publisher(s):Academic Press
Place of Publication:Amsterdam
Language:eng
Publication Date:©2014

The Physical Object

Description:xx, 624 pages, 18 unnumbered pages of plates : : illustrations, some colour ; : c4 cm

Edition Identifiers

ISBN:0128021837, 9780128021835
oclc:ocn925477701

Section I Membrane Protein Engineering, Solubilization and Purification -- 1. Multicolor Fluorescence-Based Screening Toward Structural Analysis of Multiprotein Membrane Complexes / Robert Tampe -- 1. Introduction -- 2. Production of Fluorescently Labeled TAP Complexes in P. pastoris -- 3. Production of Fluorescently Labeled TAP Complexes in Mammalian Cells -- 4. Multicolor Fluorescence-Based Screening Approaches -- 5. Summary -- Acknowledgments -- References -- 2.A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs / Mark E. Dumont -- 1. Introduction -- 2. Overall Strategy -- 3. Plasmids, Yeast Strains, and Media -- 4. Library Construction -- 5. Expression Screening and Functional Assays -- 6. Results for the Yeast a-Factor Receptor Ste2p -- Acknowledgments -- References -- 3. Membrane Preparation and Solubilization / Ankita Roy -- 1. Introduction -- 2. Membrane Preparation -- 3. Solubilization -- References.

4. Amphipathic Agents for Membrane Protein Study / Pil Seok Chae -- 1. Introduction -- 2. MP Stability in Membrane Architecture -- 3. Conventional Detergents -- 4. Novel Amphipathic Systems -- 5. Summary of Detergent Properties -- 6. Detergen…