Membrane trafficking: methods and protocols
This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal r…
Read More ↓Title Statements
- Full title :Membrane trafficking : methods and protocols / edited by Jingshi Shen.
Summary
This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Trafficking: Methods and Protocols provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs.
Read More ↓Edition Statements
- Publication Date:2022
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2022
The Physical Object
- Description:xiv, 407 pages : : illustrations (some color) ; : 26 cm.
Edition Identifiers
- ISBN:9781071622087, 1071622080
- oclc:on1295207936
- LC Classification:QH509
- Dewey Decimal:571.64
Table of Contents
Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System -- Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo -- Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH) -- Spatial and Temporal Control of Protein Secretion with Light -- Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing -- Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting -- Imaging Insulin Granule Dynamics in Human Pancreatic [beta]-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy -- Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique -- Live-Cell Super-Resolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons -- Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse -- In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion -- Imaging Single Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM) -- Localizing Proteins on Single Traff…
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