Metabolomics: Methods and Protocols

by Bhattacharya, Sanjoy K.

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Title Statements

Full title :Metabolomics : Methods and Protocols / edited by Sanjoy K. Bhattacharya.

Summary

The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson's Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested in this exciting and evolving field. .

Edition Statements

Publication Date:2019.
Edition:1st ed. 2019

Publication Statements

Publisher(s):Imprint: Humana, Springer New York
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

The Physical Object

Description:1 online resource (XV, 335 p. 82 illus., 53 illus. in color.).

Edition Identifiers

ISBN:1493994883, 9781493994885
DOI:10.1007/978-1-4939-9488-5.
oclc:on1167830023
LC Classification:QP171-177
Dewey Decimal:571.6

Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer -- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis -- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals -- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards -- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods -- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods -- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS -- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates -- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System -- Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM -- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS) -- Analysis of Microbial Siderophores b…