Methods in enzymology

Methods in enzymology

by Sehgal, Amita

Type Book
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Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides…

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Title Statements

  • Full title :Methods in enzymology. / edited by Amita Sehgal ; contributors, Ravi Allada [and fifty-three others].

Summary

Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems. Protocols are provided to assess clock fun.

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Edition Statements

  • Publication Date:©2015
  • Edition:First edition

Publication Statements

  • Publisher(s):Academic Press
  • Place of Publication:London, England
  • Language:eng
  • Publication Date:©2015

Notes

Description based upon print version of record.

2.3. Role of neuropeptides

The Physical Object

  • Description:1 online resource (432 p.).

Edition Identifiers

  • ISBN:0128033819, 9780128033814, 0128033800, 9780128033807
  • oclc:on1113102131
  • LC Classification:TP248.E5
  • Dewey Decimal:574.1/8

Table of Contents

Front Cover; Circadian Rhythms and Biological Clocks, Part B; Copyright; Contents; Contributors; Preface; Part I: Dissecting the Central Clock Circuit; Chapter One: Measuring Synchrony in the Mammalian Central Circadian Circuit; 1. Introduction; 1.1. What is synchrony?; 1.2. What is circadian synchrony?; 1.3. Goals of this review; 2. Monitoring SCN Rhythms with Cellular Resolution; 3. Isolating Data from Single Cells; 4. Defining a Rhythm; 4.1. Plotting rhythmic data for visual inspection; 5. Period Synchrony: Methods to Extract and Compare Periods Between Cells

5.1. Do cells share the same period?6. Phase Synchrony: Methods to Extract and Compare Phase Relationships Between Cells; 7. Perturbations Reveal Synchronization Mechanisms; 8. Methods Awaiting Application in Circadian Biology; 9. Step-by-Step Instructions for Measuring Synchrony in SCN Slice; 9.1. Bioluminescence recordings using a charge-coupled device camera; 9.2. Image processing; 9.3. Single-cell tracking; 9.4. Data presentation; Acknowledgments; References; Chapter Two: Patch-Clamp Electrophysiology in Drosophila Circadian Pacemaker Neurons; 1. The Drosophila Circadian Network

2. Circadian Control of Neuronal Activ…

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Subjects

Biological rhythms Circadian Rhythm Circadian rhythms enzyme Enzymes Rythmes circadiens

Writers

Sehgal, Amita Allada, Ravi Sehgal, Amita

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