Molecular chaperones: methods and protocols

by Calderwood, Stuart K. Prince, Thomas L.

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Title Statements

Full title :Molecular chaperones : methods and protocols / edited by Stuart K. Calderwood, Thomas L. Prince.

Summary

The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular Biology"!series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease

Edition Statements

Publication Date:©2011.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:©2011.

The Physical Object

Description:xviii, 321 pages : : illustrations ; : 27 cm

Edition Identifiers

ISBN:9781617792946, 1617792942, 9781617792953, 1617792950
oclc:ocn759889109
LC Classification:QP552.M64
Dewey Decimal:572.6

Targeted deletion of Hsf1, 2, and 4 genes in mice/ Xiongie Jin [and others] -- The role of heat shock factors in stress-induced transcription / Yue Zhang [and others] -- Hsp90 and client protein maturation / Natalie Wayne, Parul Mishra, and Daniel N. Bolon -- The role of p23, hop, immunophilins, and other co-chaperones in regulating Hsp90 function / Marc B. Cox and Jill L. Johnson -- Detecting Hsp90 phosphorylation / Mehdi Mollapour and Len Neckers -- Role of molecular chaperones in biogenesis of the protein kinome / Atin K. Mandal [and others] -- Nucleotide exchange factors for Hsp70 chaperones / Heike Rampelt, Matthias P. Mayer, and Bernd Bukau -- Reconstitution of CHIP E3 ubiquitin ligase activity / Hong Yu Ren [and others] -- Structure-functions of HspB1 (Hsp27) / André-Patrick Arrigo -- Combined lentiviral and RNAi technologies for the delivery and permanent silencing of the Hsp25 gene/ Punit Kaur, Ganachari M. Nagaraja, and Alexzander Asea -- Quantification of Hsp27 and Hsp70 molecular chaperone activities / Jessica Gobbo [and others] -- Measuring Hsp72 (HSPA1A) by indirect sandwich ELISA / H. Elyse Ireland and John H.H. Williams -- Analysis of heat-shock protein localisatio…

Subjects

Heat-Shock Proteins Laboratory Manual Laboratory manuals Manuels de laboratoire Molecular Chaperones Molécules chaperonnes Protéines de choc thermique