Nuclear Reprogramming: Methods and Protocols
by Beaujean, Nathalie Jammes, Hélène Jouneau, Alice
Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised …
Read More ↓Title Statements
- Full title :Nuclear Reprogramming : Methods and Protocols / edited by Nathalie Beaujean, Hélène Jammes, Alice Jouneau.
Summary
Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Read More ↓Edition Statements
- Publication Date:2015.
- Edition:2. ed
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York
- Language:eng
- Publication Date:2015.
The Physical Object
- Description:1 online resource (online resource (xi, 285 s.)) : : illustrations
Edition Identifiers
- ISBN:9781493915941, 1493915940
- oclc:ocn941370750
- Dewey Decimal:660.65
Table of Contents
1 Nuclear Transfer in the Mouse / Vincent Brochard and Zichuan Liu -- 2 Nuclear Transfer in Rabbit / Nathalie Daniel and Patrick Chesné -- 3 Nuclear Transfer in Ruminants / Joon-Hee Lee and Walid E. Maalouf -- 4 Nuclear Transfer and Transgenesis in the Pig / Mayuko Kurome, Barbara Kessler, Annegret Wuensch, Hiroshi Nagashima, and Eckhard Wolf -- 5 Embryonic Stem Cell-Somatic Cell Fusion and Postfusion Enucleation / Huseyin Sumer and Paul J. Verma -- 6 Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte / Jerome Jullien -- 7 Assessing the Quality of Donor Cells: Karyotyping Methods / Amélie Bonnet-Garnier, Anne-Clémence Veillard, Bertrand Bed'Hom, Hélène Hayes, and Janice Britton-Davidian -- 8 Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer / Eiji Mizutani, Sayaka Wakayama, and Teruhiko Wakayama -- 9 Fluorescent Immunodetection of Epigenetic Modifications on Preimplantation Mouse Embryos / Claire Boulesteix and Nathalie Beaujean -- 10 Visualization of Epigenetic Modifications in Preimplantation Embryos / Hiroshi Kimura and Kazuo Yamagata -- 11 Live Embryo Imaging to Follow Cell Cycle and Chromosomes S…
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