Optical tweezers: methods and protocols

by Gennerich, Arne

Type Book

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Title Statements

Full title :Optical tweezers : methods and protocols / edited by Arne Gennerich.

Summary

This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.

Edition Statements

Publication Date:2022
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022

Notes

Formerly CIP.

Previous edition: Boca Raton: Taylor & Francis, 2010.

The Physical Object

Description:xiv, 757 pages : : illustrations (some color) ; : 26 cm.

Edition Identifiers

ISBN:9781071622285, 1071622285
oclc:on1308629936
LC Classification:TK8360.O69
Dewey Decimal:621.36

The Invention of the Laser -- Art Ashkin and the Origins of Optical Trapping and Particle Manipulation -- Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes -- Angular Optical Trapping to Directly Measure DNA Torsional Mechanics -- Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System -- One-Dimensional STED Microscopy in Optical Tweezers -- Temperature Quantification and Temperature Control in Optical Tweezers -- High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy -- Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers -- Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay -- Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers -- Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding -- CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy -- Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Transloc…