Patch clamp electrophysiology: methods and protocols

by Bell, Damian Dallas, Mark

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Title Statements

Full title :Patch clamp electrophysiology : methods and protocols / edited by Mark Dallas, Damian Bell.

Summary

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be areference guide for current and future ion channel physiologists.

Edition Statements

Publication Date:2021

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2021

The Physical Object

Description:1 online resource (x, 333 pages) : : illustrations.

Edition Identifiers

ISBN:9781071608180, 1071608185
DOI:10.1007/978-1-0716-0818-0
oclc:on1202745913
LC Classification:QP341
Dewey Decimal:572/.437

An Introduction to Patch Clamp Recording -- Patch Clamp Technology in the 21st Century -- Heterologous Expression of Ion Channels in Mammalian Cell Lines -- Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel -- Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology -- Multi-electrode Arrays -- Utilising Automated Electrophysiological Platforms in Epilepsy Research -- Dynamic Clamp on a Windows PC -- Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing -- Bioluminescence Methodology for Ion Channel Studies -- Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons -- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method -- In Vivo Patch Clamp Studies -- In Vivo Optogenetics with Stimulus Calibration -- Single or Double Patch Clamp Recordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics -- Optogenetics and Optical Tools in Automated Patch Clamping.

Subjects

Electrophysiological Phenomena Électrophysiologie -- Manuels de labora… electrophysiology Electrophysiology -- Laboratory manuals Ion Channel Gating Laboratory Manual Laboratory manuals Manuels de laboratoire Patch-Clamp Techniques -- methods PATCH-CLAMP TECHNIQUES (ELECTROPHYSIOLO… Patch-clamp techniques (Electrophysiolo… Processus électrophysiologiques Technique du Patch-clamp -- Manuels de …