Photosynthesis research protocols

Photosynthesis research protocols

by Carpentier, Robert

Type Book
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The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are c…

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Title Statements

  • Full title :Photosynthesis research protocols / edited by Robert Carpentier.

Summary

The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology"!series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Photosynthesis Research Protocols, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process

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Edition Statements

  • Publication Date:©2011.
  • Edition:2nd ed

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York, N.Y
  • Language:eng
  • Publication Date:©2011.

The Physical Object

  • Description:1 online resource (xiv, 395 pages) : : illustrations (some color)

Edition Identifiers

  • ISBN:9781607619253, 1607619253
  • DOI:10.1007/978-1-60761-925-3
  • oclc:ocn680490599
  • LC Classification:QK882
  • Dewey Decimal:572/.46

Table of Contents

Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants -- Isolation of photosystem I submembrane fractions -- Isolation of photosystem II reaction center complexes from plants -- Methods for the isolation of functional photosystem II core particles from the cyanobacterium Synechocystis sp. PCC 6803 -- Purification and crystallization of oxygen-evolving photosystem II core complex from thermophilic cyanobacteria -- Isolation of cytochrome b6f complex from grana and stroma membranes from spinach chloroplasts -- Purification and crystallization of the cyanobacterial cytochrome B6F complex -- Purification of plastocyanin and cytochrome C6 from plants, green algae, and cyanobacteria -- Isolation and identification of chloroplast lipids -- Isolation and purification of CP43 and CP47 photosystem II proximal antenna complexes from plants -- Preparation of native and recombinant light-harvesting chlorophyll-a/b complex -- Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macro-assemblies with light-inducible structural transitions -- Protein targeting across and into chloroplast membranes -- Proteomic …

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