Plant Genotyping: Methods and protocols
The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications …
Read More ↓Title Statements
- Full title :Plant Genotyping : Methods and protocols / edited by Jacqueline Batley.
Summary
The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Read More ↓Edition Statements
- Publication Date:2015.
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York
- Language:eng
- Publication Date:2015.
The Physical Object
- Description:1 online resource (online resource (xi, 315 s.)) : : illustrations
Edition Identifiers
- ISBN:9781493919666, 1493919660
- oclc:ocn941096009
- Dewey Decimal:581.350721
Table of Contents
1 Advances in Plant Genotyping: Where the Future Will Take Us / Dhwani A. Patel, Manuel Zander, Jessica Dalton-Morgan, and Jacqueline Batley -- 2 Molecular Marker Applications in Plants / Alice C. Hayward, Reece Tollenaere, Jessica Dalton-Morgan and Jacqueline Batley -- 3 Bioinformatics: Identification of Markers from Next- Generation Sequence Data / Pradeep Ruperao and David Edwards -- 4 Molecular Marker Databases / Kaitao Lai, Michał Tadeusz Lorenc, and David Edwards -- 5 Plant Genotyping Using Fluorescently Tagged Inter-Simple Sequence Repeats (ISSRs): Basic Principles and Methodology / Linda M. Prince -- 6 SSR Genotyping / Annaliese S. Mason -- 7 Genotyping Analysis Using an RFLP Assay / Shutao Dai and Yan Long -- 8 DNA Barcoding for Plants / Natasha de Vere, Tim C.G. Rich, Sarah A. Trinder, and Charlotte Long -- 9 Multiplexed Digital Gene Expression Analysis for Genetical Genomics in Large Plant Populations / Christian Obermeier, Bertha M. Salazar-Colqui, Viola Spamer, and Rod Snowdon -- 10 SNP Genotyping by Heteroduplex Analysis / Norma Paniego, Corina Fusari, Verónica Lia, and Andrea Puebla -- 11 Application of the High-Resolution Melting Technique for Gene Mapping and SNP …
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