Plant proteostasis: methods and protocols

by Chen, Quian Lois, L. Maria Matthiesen, Rune

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Title Statements

Full title :Plant proteostasis : methods and protocols / edited by L. Maria Lois (Development Program, CRAG (CSIC-IRTA-UAB-UB) Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), Barcelona, Spain), Rune Matthiesen (Computational and Experimental Biology Group, National Health Institute Dr. Ricardo Jorge, IP, Lisbon, Portugal).

Summary

"This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken into four parts focusing on the study of ubiquitin-dependent post-translational modifications, protocols focused on Ubl post-translational modifications, protein homeostasis, and protocols for the in silico analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols aims to address next challenges in agriculture such as precision horticulture."--OCLC.

Edition Statements

Publication Date:2016.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2016.

The Physical Object

Description:1 online resource (1 recurs en línia (xi, 307 pàgines)) : : il·lustracions, gràfics.

Edition Identifiers

ISBN:9781493937592, 1493937596
oclc:ocn986665243

Conté: Approaches to Determine Protein Ubiquitination Residue Types / Qian Chen ... -- Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis Thaliana Seedlings -- Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors -- Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes -- Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants -- Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro -- Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases -- SUMO Chain Formation by Plants Enzymes -- Kinetic Analysis of Plant SUMO Conjugation Machinery -- Expression, Purification, and Enzymatic Analysis Of Plant SUMO Proteases -- Quantitative Analysis of Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy Imaging -- Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8 -- Detection of Autophagy in Plants by Fluorescence Microscopy -- Protocols for Studying Protein Stability in a Arabidopsis Protoplast Transient Expression System -- Detection and Quantification of Protein Aggregates in Plants -- Determination of Prot…