Polycomb group proteins: methods and protocols
by Bodega, Beatrice Lanzuolo, Chiara
"This volume presents the most recent technologies used in the Polycomb Group Proteins (PcG) field. Chapters detail state-of-the-art methods, creating a unique and comprehensive reference source for investigating Polycomb function in the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on …
Read More ↓Title Statements
- Full title :Polycomb group proteins : methods and protocols / edited by Chiara Lanzuolo (IBCN-CNR, Institute of Cell Biology and Neurobiology and Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", Rome, Italy), Beatrice Bodega (Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy).
Summary
"This volume presents the most recent technologies used in the Polycomb Group Proteins (PcG) field. Chapters detail state-of-the-art methods, creating a unique and comprehensive reference source for investigating Polycomb function in the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Polycomb Group Proteins: Method and Protocols aims to ensure successful results in the further study of this vital field."--OCLC.
Read More ↓Edition Statements
- Publication Date:2016.
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2016.
The Physical Object
- Description:1 online resource (1 recurs en línia (xv, 325 pàgines) :) : : il·lustracions (algunes en color).
Edition Identifiers
- ISBN:9781493963805, 1493963805
- oclc:ocn986631248
Table of Contents
Conté: Mapping the function of polycomb proteins -- Chromatin immunoprecipitation -- Chromatin preparation and chromatin immuno-precipitation from Drosophila embryos -- ChIP-seq data processing for PcG proteins and associated histone modifications -- Analysis of single-locus replication timing in asynchronous cycling cells -- Noncoding RNA interplay with the genome -- RIP : RNA immunoprecipitation -- Capture hybridization analysis of DNA targets -- Identification of RNA-protein interactions through in vitro RNA pull-down assays -- Understanding RNA-chromatin interactions using chromatin isolation by RNA purification (ChIRP) -- Analysis RNA-seq and noncoding RNA -- Dynamics of polycomb complexes -- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics -- Analysis of endogenous protein interactions of polycomb group of proteins in mouse embryonic stem cells -- Determination of polycomb group of protein compartmentalization through chromatin fractionation procedure -- Automatic segmentation method combining an active contour model and a classification technique for detecting polycomb-group proteins in high-throughput microscopy image…
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