Protein acetylation: methods and protocols

by Brosh, Robert M.

Type Book

Rate 0.0 /5

Details

Files

Reviews

Download

Title Statements

Full title :Protein acetylation : methods and protocols / edited by Robert M. Brosh, Jr.

Summary

"The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems." - Prové de l'editor.

Edition Statements

Publication Date:2019
Edition:1st ed. 2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

The Physical Object

Description:1 recurs en línia (XIII, 310 pàgines) : : 52 il·lustracions, 40 il·lustracions en color

Edition Identifiers

ISBN:9781493994342, 1493994344
DOI:10.1007/978-1-4939-9434-2
oclc:on1139495976

Conté: High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets -- Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry -- Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty -- Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC -- Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo -- Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments -- Lysine Acetylation of Proteins and its Characterization in Human Systems -- Molecular and Cellular Characterization of SIRT1 Allosteric Activators -- Assessment of SIRT2 Inhibitors in Mouse Models of Cancer -- Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair -- Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes -- Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation -- Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons -- Experimental Approaches to…