Quantitative imaging in cell biology

Quantitative imaging in cell biology

by Waters, Jennifer Catherine Wittmann, Torsten

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Title Statements

  • Full title :Quantitative imaging in cell biology / edited by Jennifer C. Waters (Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA), Torsten Wittmann (Department of Cell and Tissue Biology, University of California, San Francisco, USA).

Edition Statements

  • Publication Date:©2014
  • Edition:First edition

Publication Statements

  • Publisher(s):Academic Press
  • Place of Publication:San Diego, CA
  • Language:eng
  • Publication Date:©2014

The Physical Object

  • Description:xx, 568 pages, 20 unnumbered pages of plates : : illustrations (some colour) ; : 24 cm

Edition Identifiers

  • ISBN:9780124201385, 0124201385
  • oclc:ocn915509530
  • LC Classification:QH585
  • Dewey Decimal:570.28/2

Table of Contents

Machine generated contents note: ch. 1 Concepts in Quantitative Fluorescence Microscopy / Torsten Wittmann -- 1.1. Accurate and Precise Quantitation -- 1.2. Signal, Background, and Noise -- 1.3. Optical Resolution: The Point Spread Function -- 1.4. Choice of Imaging Modality -- 1.5. Sampling: Spatial and Temporal -- 1.6. Postacquisition Corrections -- 1.7. Making Compromises -- 1.8.Communicating Your Results -- Acknowledgment -- References -- ch. 2 Practical Considerations of Objective Lenses for Application in Cell Biology / Michael W. Davidson -- Introduction -- 2.1. Optical Aberrations -- 2.2. Types of Objective Lenses -- 2.3. Objective Lens Nomenclature -- 2.4. Optical Transmission and Image Intensity -- 2.5. Coverslips, Immersion Media, and Induced Aberration -- 2.6. Considerations for Specialized Techniques -- 2.7. Care and Cleaning of Optics -- Conclusions -- References -- ch. 3 Assessing Camera Performance for Quantitative Microscopy / Jennifer C. Waters

Note continued: 3.1. Introduction to Digital Cameras for Quantitative Fluorescence Microscopy -- 3.2. Camera Parameters -- 3.3. Testing Camera Performance: The Photon Transfer Curve -- References -- ch. 4 A Practical Guid…

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Subjects

Cytofluorimétrie Cytofluorometry Imagerie en biologie Imaging systems in biology Microscopie microscopy

Writers

Waters, Jennifer Catherine Wittmann, Torsten

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