Seed dormancy: methods and protocols

by Kermode, Allison R.

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Title Statements

Full title :Seed dormancy : methods and protocols / edited by Allison R. Kermode.

Summary

"Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world's food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8'-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls."--Publisher's description

Edition Statements

Publication Date:©2011.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:©2011.

The Physical Object

Description:xvi, 423 pages : : illustrations ; : 27 cm

Edition Identifiers

ISBN:9781617792304, 1617792306, 9781617792311, 1617792314
oclc:ocn733237606
LC Classification:QK661
Dewey Decimal:575.6/8

Introduction -- Breaking seed dormancy and standardizing seed dormancy research -- Hormone analyses -- Gene, RNA, and protein analyses -- Epigenetic changes associated with seed dormancy -- Spectroscopic and whole tissue analyses -- Analyes of reactive oxygen species, protein damage and repair -- Bioinformatic resources.

Subjects

Laboratory Manual Laboratory manuals Manuels de laboratoire Plant Dormancy seed dormancy Seeds -- Dormancy Seeds -- growth & development Seeds -- Laboratory manuals. -- Dormancy Seeds -- physiology Semences -- Dormance Semences -- Manuels de laboratoire. -- …