Skeletal development and repair: methods and protocols

by Hilton, Matthew J.

Type Book

Rate 0.0 /5

Details

Files

Reviews

Download

Title Statements

Full title :Skeletal development and repair : methods and protocols / edited by Matthew J. Hilton.

Summary

This second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.

Edition Statements

Publication Date:2021
Edition:Second edition

Publication Statements

Publisher(s):Humana Press, Springer
Place of Publication:New York
Language:eng
Publication Date:2021

The Physical Object

Description:1 online resource

Edition Identifiers

ISBN:9781071610282, 1071610287
DOI:10.1007/978-1-0716-1028-2
oclc:on1225069307
LC Classification:QL821
Dewey Decimal:573.7/6

Overview of Skeletal Development -- Overview of Skeletal Repair (Fracture Healing and Its Assessment) -- Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research -- Generation of Closed Transverse Fractures in Small Animals -- A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes -- Surgical Induction of Posttraumatic Osteoarthritis in the Mouse -- Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton -- Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development -- Murine Limb Explant Cultures to Assess Cartilage Development -- Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair -- MicroCT for Scanning and Analysis of Mouse Bones -- Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties -- Phenotyping Intact Mouse Bones using Bone CLARITY -- Processing and Sectioning Undecalcified Murine Bone Specimens -- Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Hard Tissues Using Kawamoto's Film Method (2020) -- Demineralized Murine Skeletal Histology -- Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Chan…

Subjects

Bones -- Growth Bones -- Laboratory manuals. -- Growth Laboratory Manual Laboratory manuals Manuels de laboratoire Mice as laboratory animals Orthopedics Os -- Manuels de laboratoire. -- Croiss… Skeleton Skeleton -- Laboratory manuals Souris (Animal de laboratoire) Squelette -- Manuels de laboratoire