Skin tissue engineering: methods and protocols

by Biedermann, Thomas Böttcher-Haberzeth, Sophie

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Title Statements

Full title :Skin tissue engineering : methods and protocols / edited by Sophie Böttcher-Haberzeth, Thomas Biedermann.

Summary

"This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine." - Prové de l'editor.

Edition Statements

Publication Date:2019
Edition:1st ed. 2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

The Physical Object

Description:1 recurs en línia (XII, 263 pàgines) : : 79 il·lustracions, 66 il·lustracions en color

Edition Identifiers

ISBN:9781493994731, 1493994735
DOI:10.1007/978-1-4939-9473-1
oclc:on1139472930

Conté: Part 1 : Isolation and culture of skin cells -- Chap 1: Isolation and Culture of Human Keratinocytes -- Chap 2 : Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture -- Chap 3 : Isolation and Culture of Epidermal Melanocytes -- Chap 4: Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor -- Chap 5 : Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells -- Chap 6 : Isolation and Culture of Human Dermal Fibroblasts -- Chap 7: Isolation and Culture of Human Dermal Microvascular Endothelial Cells -- Chap 8: Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue -- Part 2 : Tissue engineering skin and engineered skin models -- Chap 9 : Engineering a Multi-Layered Skin Equivalent: the Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility -- Chap 10 : Three-dimensional Epidermal Model from Human Hair follicle-derived Keratinocytes -- Chap 11 : Fabrication of a Co-Culture System with Human Sweat Gland Derived Cells and Peripheral Nerve Cells -- Chap 12 : Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblas…