SMC complexes: methods and protocols

by Badrinarayanan, Anjana

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Title Statements

Full title :SMC complexes : methods and protocols / edited by Anjana Badrinarayanan.

Summary

"This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SMC Complexes: Methods and Protocols will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA. Chapters 3, 10 and 19 are available Open Access under a CC BY 4.0 license via link.springer.com." - Prové de l'editor.

Edition Statements

Publication Date:2019
Edition:1st ed. 2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

The Physical Object

Description:1 recurs en línia (XIII, 336 pàgines) : : 91 il·lustracions, 55 il·lustracions en color

Edition Identifiers

ISBN:9781493995202, 1493995200
DOI:10.1007/978-1-4939-9520-2
oclc:on1139488011

Conté: Using Cell Cycle Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosome (SMC) Complexes In Vivo -- Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System -- Efficient Depletion of Fission Yeast Condensin by Combined Transcriptional Repression and Auxin-Induced Degradation -- Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts -- High-Throughput Allelic Replacement Screening in Bacillus subtilis -- Identifying Functional Domains in Subunits of Structural Maintenance of Chromosome (SMC) Complexes by Transposon Mutagenesis Screen in Yeast -- Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes -- Knocking in Multi-Functional Gene Tags into SMC Complex Subunits Using Gene Editing -- Chromosome Conformation Capture with Deep Sequencing to Study the Roles of the Structural Maintenance of Chromosomes Complex In Vivo -- Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation -- Analysis of Cohesin Association to Newly Replicated DNA through Nascent Strand Binding Assay (NSBA) -- Preparation of Cell Cycle S…