Stem Cell Mobilization: Methods and Protocols

by Klein, Gerd

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Title Statements

Full title :Stem Cell Mobilization : Methods and Protocols.

Summary

This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Mobilization: Methods and Protocols is an ideal guide for all laboratories in their endeavors to decipher the mechanisms of stem cell mobilization, ultimately helping stem cell donors and patients around the world.

Edition Statements

Publication Date:2019.

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

Notes

3.1.3 Preparation of Lower Well Contents of the AP48 Transwell Chemotaxis Chamber

The Physical Object

Description:1 online resource (250 pages)

Edition Identifiers

ISBN:9781493995745, 149399574X, 9781493995738, 1493995731, 9781493995752, 1493995758, 9781493995769, 1493995766
DOI:10.1007/978-1-4939-9574-5
oclc:on1122917740
LC Classification:QH301-705
Dewey Decimal:571.6

Intro; Preface; Contents; Contributors; Chapter 1: Evolution of Peripheral Blood Stem Cell Transplantation; 1 Introduction; 2 Development of Bone Marrow Transplantation; 3 Trafficking of HSC in Peripheral Blood; 4 The First Blood Stem Cell Transplantation: Need Is the Mother of Invention; 5 Bone Marrow Versus Peripheral Blood as the Source of Stem Cells; 6 Challenges in Mobilization of PBSC; 7 ``Poor Mobilizers;́́ 8 Future Perspectives of Hematopoietic Stem Cell Transplantation; References; Chapter 2: In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents; 1 Introduction

2 Materials2.1 Functionalization of Supported Membranes; 2.2 Isolation of Human HSPC; 2.3 Quantifying Significance of Cell Adhesion; 3 Methods; 3.1 Cleaning of Glass Slides; 3.2 Supported Membrane Preparation; 3.3 Functionalization with Ligands; 3.4 Isolation of Human HSPC; 3.5 Incubation with Mobilizing Agents; 3.6 Quantifying Significance of Cell Adhesion; 3.7 Extraction of Dynamic Cellular Phenotypes (Shape); 3.8 Extraction of Dynamic Cellular Phenotypes (Motion); 3.9 Final Remarks; 4 Notes; References; Chapter 3: Colony Formation: An Assay of Hematopoietic Progenitor Cells; 1 Introduction

2 Materials…