Stem cell mobilization: methods and protocols

by Klein, Gerd Wuchter, Patrick

Type Book

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Title Statements

Full title :Stem cell mobilization : methods and protocols / Gerd Klein, Patrick Wuchter.

Summary

"This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. " -- Prové de l'editor.

Edition Statements

Publication Date:©2019

Publication Statements

Publisher(s):Humana
Place of Publication:New York
Language:eng
Publication Date:©2019

Notes

Conté: 1. Evolution of Peripheral Blood Stem Cell Transplantation Anthony D. Ho 2. In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents Motomu Tanaka 3. Colony Formation: An Assay of Hematopoietic Progenitor Cells Romy Kronstein-Wiedemann and Torsten Tonn 4. Mobilization and Collection of Peripheral Blood Stem Cells in Adults: Focus on Timing and Benchmarking Katharina Kriegsmann and Patrick Wuchter 5. Transmigration Assays for the Determination of Molecular Interactions between Hematopoietic Stem Cells and Niche Cells Reinhard Henschler and Rudolf Richter 6. Microfluidic Shear Force Assay to Determine Cell Adhesion Forces Julia Hümmer, Julian Koc, Axel Rosenhahn, and Cornelia Lee-Thedieck 7. A Microcavity Array-Based 3D-Model System of the Hematopoietic Stem Cell Niche Eric Gottwald, Cordula Nies, Patrick Wuchter, Rainer Saffrich, Roman Truckenmüller, and Stefan Giselbrecht 8. Migration Assay for Leukemic Cells in a 3D Matrix toward a Chemoattractant Sabrina Zippel, Annamarija Raic, and Cornelia Lee-Thedieck 9. Intravital Imaging of Blood Flow and HSPC Homing in Bone Marrow Microvessels Jonas Stewen and Maria Gabriele Bixel 10. Assessing Cellular Hypoxic Status In Situ within the Bone Marrow Microenvironment Ute Suessbier and César Nombela-Arrieta 11. Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry Angela Jacobi, Philipp Rosendahl, Martin Kräter, Marta Urbanska, Maik Herbig, and Jochen Guck 12. Assessment of Proteolytic Activities in the Bone Marrow Microenvironment Andreas Maurer, Gerd Klein, and Nicole D. Staudt 13. Analysis of the Complement Cascade Activation during Mobilization of Hematopoietic Stem/Progenitor Cells Anna I. Grabowska and Jakub M. Hawryluk 14. A Freezing Protocol for Hematopoietic Stem Cells Petra Pavel and Sascha Laier 15. Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays Yu Wei Zhang and Nina Cabezas Wallscheid 16. Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells Martina Konantz, Joëlle S. Müller, and Claudia Lengerke 17. Statistical and Mathematical Modeling of Spatiotemporal Dynamics of Stem Cells Walter de Back, Thomas Zerjatke, and Ingo Roeder.