Stem cell niche: methods and protocols

by Turksen, Kursad

Type Book

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Title Statements

Full title :Stem cell niche : methods and protocols / edited by Kursad Turksen.

Summary

"This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the first edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. " -- Prové de l'editor.

Edition Statements

Publication Date:2019
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

Notes

Conté: 1. In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres Sofie Mohlin and Laura Kerosuo 2. Analysis of Hematopoietic Niche in the Mouse Embryo Keai Sinn Tan, Nathalie Brouard, and Daisuke Sugiyama 3. Functional Assays of Stem Cell Properties Derived from Different Niches Beatriz de Lucas, Laura M. Pérez, and Beatriz G. Gálvez 4. Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche Aviva J. Goel and Robert S. Krauss 5. Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment Madeline Campbell, Mamta Chabria, Gemma A. Figtree, Liudmila Polonchuk, and Carmine Gentile 6. Isolation, Propagation, and Clonogenicity of Intestinal Stem Cells Prashanthi Ramesh, Aleksandar Buryanov Kirov, David Johannes Huels, and Jan Paul Medema 7. Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells Mary C. Morton, Victoria N. Neckles, and David M. Feliciano 8. Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao, and Yan Jin 9. Three-Dimensional Co-Culture of Human Hematopoietic Stem/Progenitor Cells and Mesenchymal Stem/Stromal Cells in a Biomimetic Hematopoietic Niche Microenvironment Marta H.G. Costa, Tiago S. Monteiro, Susana Cardoso, Joaquim M.S. Cabral, Frederico Castelo Ferreira, and Cláudia L. da Silva 10. Investigating the Vascular Niche: Three-Dimensional Co-Culture of Human Skeletal Muscle Stem Cells and Endothelial Cells Claire Latroche, Michèle Weiss-Gayet, and Bénédicte Chazaud 11. Identification and Characterization of Stem Cells in Oral Cancer Sujit K. Bhutia, Prajna P. Naik, Prakash P. Praharaj, Debasna P. Panigrahi, Chandra S. Bhol, Kewal K. Mahapatra, Sarbari Saha, and Srimanta Patra 12. Determining Competitive Potential of Bone Metastatic Cancer Cells in the Murine Hematopoietic Stem Cell Niche Sun H. Park, Matthew R. Eber, Russell S. Taichman, and Yusuke Shiozawa 13. Spatial Genomic Analysis: A Multiplexed Transcriptional Profiling Method that Reveals Subpopulations of Cells within Intact Tissues Antti Lignell and Laura Kerosuo 14. 3D Culture of Mesenchymal Stem Cells in Alginate Hydrogels Sílvia J. Bidarra and Cristina C. Barrias 15. Isolation and Identification of Murine Bone Marrow-Derived Macrophages and Osteomacs from Neonatal and Adult Mice Joydeep Ghosh, Safa F. Mohamad, and Edward F. Srour.