The Mitotic spindle: methods and protocols

by Balchand, Sai K. Chang, Paul Ohi, Ryoma

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Title Statements

Full title :The Mitotic spindle : methods and protocols / edited by Paul Chang (Massachusetts Institute of Technology, Cambridge, MA, USA), Ryoma Ohi (Department of Cell & Developmental Biology, Vanderbilt University, Nashville, TN, USA).

Summary

"This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field"--OCLC.

Edition Statements

Publication Date:2016.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2016.

The Physical Object

Description:1 online resource (1 recurs en línia (xii, 424 pàgines) :) : : il·lustracions (algunes en color).

Edition Identifiers

ISBN:9781493935420, 1493935429
oclc:ocn970411520

Conté: Using Fluorescence Microscopy to Study Mitosis / Sai K. Balchand ... -- Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation -- Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts -- Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro -- Imaging and Quantifying the Dynamics of [gamma]-Tubulin at Microtubule Minus Ends in Mitotic Spindles -- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy -- Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size -- In Vitro Kinetochore Assembly -- Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes -- Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells -- Studying Kinetochores In Vivo Using FLIM-FRET -- Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies -- A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion -- Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regu…