The nuclear envelope: methods and protocols

by Collas, Philippe Korfali, Nadia Schirmer, Eric C. Shackleton, Sue

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Title Statements

Full title :The nuclear envelope : methods and protocols / edited by Sue Shackleton (Department of Biochemistry, University of Leicester, Leicester, UK), Philippe Collas (Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway), Eric C. Schirmer (Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK).

Summary

"This volume provides a wide rangeof protocols used in studying the nuclear envelope, with special attention tothe experimental adjustments that may be required to successfully investigatethis complex organelle in cells from various organisms. T he Nuclear Envelope:Methods and Protocols is divided into five sections: Part I - Nuclear EnvelopeIsolation; Part II - Nuclear Envelope Protein Interactions, Localization, andDynamics; Part III - Nuclear Envelope Interactions with the Cytoskeleton; PartIV - Nuclear Envelope-Chromatin Interactions; and Part V - Nucleo-CytoplasmicTransport. Many of the modifications discussed in this book have only beencirculated within laboratories that have conducted research in this field formany years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, listsof the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field."--Springer.

Edition Statements

Publication Date:2016.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:spa
Publication Date:2016.

The Physical Object

Description:1 online resource (1 recurs en línia (xiv, 523 pàgines) :) : : il·lustracions (algunes en color).

Edition Identifiers

ISBN:9781493935307, 1493935305
oclc:ocn970482224

Conté: Isolation, proteomic analysis, and microscopy confirmation of the liver nuclear envelope proteome / Nadia Korfali ... -- Exploring the protein composition of the plant nuclear envelope -- High-efficiency isolation of nuclear envelope protein complexes from trypanosomes -- Superresolution microscopy of the nuclear envelope and associated proteins -- Analyses of the dynamic properties of nuclear lamins by fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) -- Probing protein distribution along the nuclear envelope in vivo by using single-point FRAP -- Use of two-photon FRET-FLIM to study protein interactions during nuclear envelope fusion in vivo and in vitro -- Identifying protein-protein associations at the nuclear envelope with BioID -- In situ detection of interactions between nuclear envelope proteins and partners -- Methods for single-cell pulse-chase analysis of nuclear components -- Analysis of nuclear lamina proteins in myoblast differentiation by functional complementation -- Analysis of meiotic telomere behavior in the mouse -- Identification and validation of putative nesprin variants -- Detection of diverse and high m…