The Nucleolus: methods and protocols

by Németh, Attila Sluis, Marjolein van

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Title Statements

Full title :The Nucleolus : methods and protocols / edited by Attila Németh.

Summary

"This volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. Chapters cover a diversity of protocols that include imaging of the nucleolus, analysis of ribosomal RNA transcription and processing, and genomics and proteomics of the nucleolus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practical handbook to facilitate the investigation of this nuclear compartment at the advanced level."--OCLC.

Edition Statements

Publication Date:2016

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2016

The Physical Object

Description:1 recurs en línia (xi, 279 pàgines) : : il·lustracions (en color), gràfics, taules

Edition Identifiers

ISBN:9781493937929, 1493937928
oclc:ocn986544224

Conté: The Relationship between Human Nucleolar Organizer Regions and Nucleoli, Probed by 3D-Immunofish / Marjolein van Sluis ... -- Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces Cerevisiae -- High-Throughput Live-Cell Microscopy Analysis of Chromosome Domain -- Nucleolus Associations in S. Cerevisiae -- Quantitative Immunofluorescence Analysis of Nucleolus-Associated Chromatin -- Visualization of The Nucleolus in Living Cells with Cell-Penetrating Fluorescent Peptides -- Purification of S. Cerevisiae RNA Polymerase I for Structural and Functional Studies -- Analysis of S. Cerevisiae RNA Polymerase I Transcription In Vitro -- Preparation of Chromatin Templates to Study RNA Polymerase I Transcription In Vitro -- Measurement of Rrna Synthesis and Processing by 4su-Tagging -- Metabolic Labeling in the Study of Mammalian Ribosomal RNA Synthesis -- Quantitative Northern Blot Analysis of Mammalian Rrna Processing -- Complete Sequence Construction of the Highly Repetitive Ribosomal RNA Gene Repeats in Eukaryotes using Whole Genome Sequence Data -- Analysis of Rdna Methylation in Arabidopsis By 2D Gel Electrophoresis -- Fluorescence-Activated Sortin…