The Nucleus

by Hancock, Ronald

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Title Statements

Full title :The Nucleus / edited by Ronald Hancock.

Summary

"This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study. Like the previous editions, this book features contributions from top experts in their respective fields. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, The Nucleus, Third Edition will help students and postdocs become aware of vital available methodologies when planning their new projects." -- Prové de l'editor.

Edition Statements

Publication Date:2020
Edition:3rd ed. 2020

Publication Statements

Publisher(s):Imprint: Humana, Springer US
Place of Publication:New York, NY
Language:eng
Publication Date:2020

The Physical Object

Description:1 recurs en línia (1 recurs en línia (XI, 220 pàgines) :) : 42 il·lustracions, 30 il·lustracions en color.

Edition Identifiers

ISBN:9781071607633, 1071607634
DOI:10.1007/978-1-0716-0763-3
oclc:on1251798172
LC Classification:P2E

Conté: Live-Cell Imaging and Analysis of Nuclear Body Mobility -- Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell -- Visualizing Chromatin Modifications in Isolated Nuclei -- Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture -- Studying Proton Gradients Across the Nuclear Envelope -- COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes -- Genome-Wide Mapping of UV-Induced DNA Damage with CPD-Seq -- AP-Seq: A Method to Measure Apurinic Sites and Small Base Adducts Genome-Wide -- Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID -- Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions -- Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres -- Macromolecular Crowding Measurements with Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells -- Analysis of a Nuclear Intrinsically Disordered Proteome -- Timing of Cytosine Methylation on Ne…