Vaccine adjuvants: methods and protocols

by Fox, Christopher B.

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Title Statements

Full title :Vaccine adjuvants : methods and protocols / edited by Christopher B. Fox, Infectious Disease Research Institute, Seattle, WA, USA ; Department of Global Health, University of Washington, Seattle, WA, USA.

Summary

"This collection seeks to elucidate the practical methods necessary for successful adjuvant development, with a particular focus on the synthesis, formulation, manufacturing, and characterization aspects involved. Beginning with an overview and a case study, the book then delves into in silico design, chemical synthesis, biosynthesis, and/or purification from natural raw materials of specific adjuvant molecules, adjuvant formulation approaches, the analytical characterization of adjuvant formulations and adjuvant-containing vaccines, as well as the biological characterization of vaccine adjuvant activity, including in vitro and in vivo approaches, to measure innate and adaptive immune responses. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Vaccine Adjuvants: Methods and Protocols aims to facilitate vaccine adjuvant product development efforts, making them more accessible, manufacturable, and better characterized.

Edition Statements

Publication Date:2017.

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2017.

The Physical Object

Description:1 online resource (1 recurs en línia (xii, 372 pàgines) :) : : il·lustracions (algunes en color).

Edition Identifiers

ISBN:9781493964451, 1493964453
oclc:ocn994718190

Conté: Overview of Vaccine Adjuvants: Introduction, History, and Current Status -- Development of the CpG Adjuvant 1018: A Case Study -- Syntheses of Human TLR8-Specific Small-Molecule Agonists -- Semisynthesis of Analogues of the Saponin Immunoadjuvant QS-21 -- QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation into Liposomes -- Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reverse-Phase Silica Gel Chromatography -- Biosynthetic Approaches to Squalene Production: The Case of Yeast -- In Silico Adjuvant Design and Validation -- Manufacturing Methods for Liposome Adjuvants -- Synthesis of Lymph Node-Targeting Adjuvants -- Preparing an Adjuvanted Thermoresponsive Gel Formulation for Sublingual Vaccination -- Manufacture of Oil-in-Water Emulsion Adjuvants -- Methods to Prepare Aluminum Salt-Adjuvanted Vaccines -- Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines -- Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze Dried Product -- Stressed Stability Techniques for Adjuvant Formulations -- Particle Sizing of Nanoparticle Adjuvant Formulations by Dynamic Ligh…