Biopesticides have seen a recent growth, which is partially due to increased advances in biotechnological tools for pest control. However, the growth has been largely spurred by the growing needs for new tools to fight pesticide resistance and safer and more benign means of pest…
This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TA…
This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TA…
A practical resource for everyone involved in the gene therapy field and in the design of effective gene delivery systems, this volume presents an overview and update of recent advances in the field of non-viral methods for the in vivo transfer of therapeutic genes to biological…
The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods a…
Carrying on the high standards of the much-praised first edition (Durum and Muegge, Cytokine Knockouts, 1998), Giamila Fantuzzi and a panel of experts have generated completely new chapters to reflect the use of many novel mouse strains and the hundreds of recent studies on cyto…
This volume presents the state-of-the-art description of the current understanding of the biology of somatostatin including its gene regulation, prosomatostatin processing, cellular effects, somatostatin receptor subtypes, mechanism of action, signal transduction, regulation of …
Gene Targeting and Embryonic Stem Cells
by Thomson, Alison, Mcwhir, Jim
fromAdvanced methods
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene tar…
This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells.