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This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, charact…
This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantific…
This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantific…
This detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF- ) and other cell signaling knowledge. Many chapters cover quantitative assays for TGF- signaling studies, with others…
"The field of genome editing has progressed incredibly in the last few years mainly due to the emergence of versatile genome editing tools such as endonuclease-based systems which can be used to efficiently edit gene sequences in a targeted fashion. The CRISPR/Cas9 genome editin…
"The field of genome editing has progressed incredibly in the last few years mainly due to the emergence of versatile genome editing tools such as endonuclease-based systems which can be used to efficiently edit gene sequences in a targeted fashion. The CRISPR/Cas9 genome editin…
This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) …
This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion b…
Edycja genów: władza nad ewolucją
by Doudna, Jennifer A., Sternberg, Samuel H., Tuz, Adam
0
Type: Book
This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic informat…
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusi…
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusi…
This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The collection discusses vectors other than the tradi…
This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The collection discusses vectors other than the tradi…
"Bringing together historical and ethical insights on the revolutionary, Nobel prize winning CRISPR/Cas9 gene editing technology, this accessible book examines the history of human attempts to understand and control our evolution, how the CRISPR/Cas9 technology works and what it…
"Bringing together historical and ethical insights on the revolutionary, Nobel prize winning CRISPR/Cas9 gene editing technology, this accessible book examines the history of human attempts to understand and control our evolution, how the CRISPR/Cas9 technology works and what it…
Genome engineering via CRISPR-Cas9 system
0
Type: Book
"This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifi…
The use of CRISPR/Cas technology for genome editing suggests many potential applications, including the alteration of the germline of humans, animals and food crops. The speed and efficiency of the CRISPR/Cas system make it a potentially useful system for gene therapy. In this v…