CRISPR guide RNA design: methods and protocols

by Ferry, Quentin R. V. Fulga, Tudor A. Knapp, David J. H. F.

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Title Statements

Full title :CRISPR guide RNA design : methods and protocols / edited by Tudor A. Fulga, David J. H. F. Knapp, and Quentin R. V. Ferry

Summary

"This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications."--Page 4 de la couverture

Edition Statements

Publication Date:©2021

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:©2021

Notes

Comprend un index

The Physical Object

Description:x, 286 pages : : illustrations en partie en couleur ; : 26 cm

Edition Identifiers

ISBN:9781071606865 (rel), 1071606867 (rel)
oclc:on1225207715

Subjects

ARN ARN -- génétique ARN -- Manuels de laboratoire. -- Éditi… Clustered Regularly Interspaced Short P… CRISPR (génétique) Édition des ARN -- Manuel de laboratoire Interférence par ARN Laboratory Manual Laboratory manuals Manuels de laboratoire RNA Editing RNA editing -- Laboratory manuals Systèmes CRISPR-Cas