Agrobacterium Protocols

by Wang, Kan

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Title Statements

Full title :Agrobacterium Protocols / edited by Kan Wang.

Summary

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Edition Statements

Publication Date:2015.
Edition:3. ed

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2015.

The Physical Object

Description:1 online resource (online resource (xiv, 368, 1 s.)) : : illustrations

Edition Identifiers

ISBN:9781493916955, 1493916955
oclc:ocn941370824
Dewey Decimal:631.5233

PART I VECTORS AND MODEL PLANTS -- 1 Gateway®-Compatible Plant Transformation Vectors / Mark A. Smedley and Wendy A. Harwood -- 2 Brachypodium distachyon / Jennifer N. Bragg, Amy Anderton, Rita Nieu, and John P. Vogel -- 3 Medicago truncatula Transformation Using Cotyledonary Explants / Elane Wright and Zeng-Yu Wang -- 4 Medicago truncatula Transformation Using Leaf Explants / Viviane Cosson, Alexis Eschstruth, and Pascal Ratet -- 5 Setaria viridis / Joyce Van Eck and Kerry Swartwood -- PART II CEREAL PLANTS -- 6 Barley (Hordeum vulgare L.) Transformation Using Immature Embryos / Cornelia Marthe, Jochen Kumlehn, and Goetz Hensel -- 7 Barley (Hordeum vulgare L.) Transformation Using Embryogenic Pollen Cultures / Ingrid Otto, Andrea Müller, and Jochen Kumlehn -- 8 Maize (Zea mays L.) / Bronwyn Frame, Katey Warnberg, Marcy Main, and Kan Wang -- 9 Maize, Tropical (Zea mays L.) / Shireen K. Assem -- 10 Finger millet [Eleusine coracana (L.) Gaertn.] / Stanislaus Antony Ceasar and Savarimuthu Ignacimuthu -- 11 Oat (Avena sativa L.) / Sebastian Gasparis and Anna Nadolska-Orczyk -- 12 Rice, Indica (Oryza sativa L.) / Yukoh Hiei, Yuji Ishida, and Toshihiko Komari -- 13 Rice, Japonica (Oryza…

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