Intermediate filament proteins

Intermediate filament proteins

by Omary, M. Bishr

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Title Statements

  • Full title :Intermediate filament proteins / edited by M. Bishr Omary, Ronald K. H. Liem ; contributors, Cedric Badowski [and one hundred four others].

Edition Statements

  • Publication Date:©2016
  • Edition:First edition

Publication Statements

  • Publisher(s):Academic Press
  • Place of Publication:Amsterdam, [Netherlands]
  • Language:eng
  • Publication Date:©2016

Notes

Description based upon print version of record.

1.2. Available Tools and Major Limitations for the Study of IF Protein PTMs

The Physical Object

  • Description:1 online resource (857 p.).

Edition Identifiers

  • ISBN:0128034912, 9780128034910, 012803470X, 9780128034705
  • oclc:on1113653388
  • LC Classification:TP248.27.F86
  • Dewey Decimal:660.62

Table of Contents

Front Cover; Intermediate Filament Proteins; Copyright; Dedication; Contents; Contributors; Preface; References; Acknowledgments; Part I: General Methods to Study IF Proteins; Chapter One: How to Study Intermediate Filaments in Atomic Detail; 1. Introductory Remarks; 2. Bioinformatics; 3. X-ray Crystallography; 3.1. Design of IF Protein Fragments for Crystallization; 3.2. Protein Expression; 3.3. Purification; 3.4. Crystallization; 3.5. X-ray Data Collection; 3.6. Phasing by Molecular Replacement; 3.7. Experimental Phasing Using Heavy Atoms; 3.8. Structure Refinement and Validation

3.9. Limitations and Pitfalls3.10. Obtained Structures and Impact; 4. Electron Paramagnetic Resonance with Site-Directed Spin Labeling; 4.1. Principle; 4.2. Sample Preparation and Measurements; 4.3. Data Interpretation and Impact; 4.4. Limitations and Outlook; 5. Concluding Remarks; Acknowledgments; References; Chapter Two: Mechanical Properties of Intermediate Filament Proteins; 1. Introduction; 2. Viscoelasticity of Purified IFs In Vitro; 3. IFs and the Mechanical Properties of Cells; 3.1. Type III IF: Vimentin and Desmin; 3.1.1. Knockout Models of Vimentin

3.1.2. Drugs and Proteins Disrupting Vimen…

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Subjects

Champignons filamenteux -- Biotechnolog… Filamentous fungi -- Biotechnology Intermediate Filament Proteins Protéines des filaments intermédiaires

Writers

Omary, M. Bishr Badowski, Cedric Liem, Ronald K. H. Omary, M. Bishr

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