Leptospira spp: Methods and Protocols

by Koizumi, Nobuo Picardeau, Mathieu

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Title Statements

Full title :Leptospira spp. : Methods and Protocols / edited by Nobuo Koizumi, Mathieu Picardeau.

Summary

"This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms." -- Prové de l'editor.

Edition Statements

Publication Date:2020
Edition:1st ed. 2020

Publication Statements

Publisher(s):Imprint: Humana, Springer US
Place of Publication:New York, NY
Language:eng
Publication Date:2020

The Physical Object

Description:1 recurs en línia (1 recurs en línia (X, 291 pàgines) :) : 66 il·lustracions, 56 il·lustracions en color.

Edition Identifiers

ISBN:9781071604595, 1071604597
DOI:10.1007/978-1-0716-0459-5
oclc:on1251788627
LC Classification:P2E

Conté: Isolation and Culture of Leptospira from Clinical and Environmental Samples -- Core Genome Multi-Locus Sequence Typing Analyses of Leptospira spp. using the Bacterial Isolate Genome Sequence Database -- Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira -- Cell Enumeration of Leptospira by Flow Cytometry -- The Single-Step Method of RNA Purification Applied to Leptospira -- Purification of LPS from Leptospira -- Isolation, Purification, and Characterization of Leptophages -- Creating a Library of Random Transposon Mutants in Leptospira -- Transposon Sequencing in Leptospira interrogans -- Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically inactive Cas9 (dCas9) -- Leptospira spp. Toolbox for Chemotaxis Assay -- In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography -- Measurement of the Cell-Body Rotation of Leptospira -- In Vivo Imaging of Bioluminescent Leptospires -- Cell Monolayer Translocation Assay -- Cell Adhesion Assay to Study Leptospiral Proteins: An Approach to Investigate Host-Pathogen Interaction -- Complement Resistance Assays -- Evaluation of Intracellular Trafficking in Macrophages -- Biofilm Formatio…