Methods in enzymology

by Feig, Andrew L.

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Title Statements

Full title :Methods in enzymology. / edited by Andrew L. Feig.

Edition Statements

Publication Date:©2016
Edition:First edition

Publication Statements

Publisher(s):Academic Press is an imprint of Elsevier
Place of Publication:Cambridge, MA, USA
Language:eng
Publication Date:©2016

The Physical Object

Description:xix, 493 pages, 16 unnumbered pages of plates : : illustrations (some color) ; : 24 cm.

Edition Identifiers

ISBN:0128029064, 9780128029060
oclc:ocn948332569
LC Classification:QH324.9.C3
Dewey Decimal:572/.43

Isothermal titration calorimetry measurements of metal ions binding to proteins -- Assess coupled protein folding and binding through temperature-dependent isothermal titration calorimetry -- Fragment-based screening for enzyme inhibitors using calorimetry -- Measuring multivalent binding interactions by isothermal titration calorimetry -- Calorimetric and spectroscopic analysis of the thermal stability of short duplex DNA-containing sugar and base-modified nucleotides -- Calorimetric quantification of cyclodextrin-mediated detergent extraction for membrane-protein reconstitution -- Extending ITC to kinetics with KinlTC -- Measuring the kinetics of molecular association by isothermal titration calorimetry -- Isothermal titration calorimetry to characterize enzymatic reactions -- Avoiding buffer interference in ITC experiments: a case study from the analysis of entropy-driven reactions of glucose-6-phosphate dehydrogenase -- ITC methods for assessing buffer/protein interactions from the perturbation of steady-state kinetics: a reactivity study of homoprotocatechuate 2,3-dioxygenase -- Modern analysis of protein folding by differential scanning calorimetry -- A guide to differential…