Phytochromes: methods and protocols

by Hiltbrunner, Andreas

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Title Statements

Full title :Phytochromes : methods and protocols / edited by Andreas Hiltbrunner.

Summary

This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phytochrome binding proteins using yeast two hybrid assays, in vitro pull down, and co-immunoprecipitation from stable transgenic lines; investigations into phosphorylation, SUMOylation, and ubiquitination of phytochromes and other proteins; a look at how in vivo spectroscopy can be used to measure Pfr levels of phytochrome in planta; identifying hormonal action and co-action in light-regulated processes; and ways to use the CRISPR/Cas9 system to generate Physcomitrella mutant lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Edition Statements

Publication Date:2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2019

The Physical Object

Description:1 online resource (xii, 278 pages) : : illustrations (black and white, and colour)

Edition Identifiers

ISBN:9781493996124, 1493996126
oclc:on1111580527
LC Classification:QK898.P67
Dewey Decimal:571.82

Investigation of Light-Regulated Protein-Protein Interactions using Yeast Two-Hybrid Assays -- Rapid Examination of Phytochrome-PHYTOCHROME INTERACTING FACTOR (PIF) Interaction by In Vitro Co-Immunoprecipitation Assay -- Characterization of Light-Regulated Protein-Protein Interactions by In Vivo Co-Immunoprecipitation (Co-IP) Assays from Plant -- Detection of Phytochrome Phosphorylation in Plants -- Detection of SUMOylated Phytochromes in Plants -- Approaches to Identify Protein Ubiquitination Sites in Plants -- Expression, Purification, and Spectral Characterization of Phytochromes -- In Vivo Spectroscopy -- Basic Phytochrome B Calculations -- Quantitative Analysis of Photobodies -- Regulation of Alternative Splicing by Phytochrome -- Phytochrome-Regulation of Seed Germination -- Shade Avoidance and Neighbor Detection -- A Simple Method for Quantification of Protochlorophyllide in Etiolated Arabidopsis Seedlings -- Measuring Phytochrome-Dependant Light-Input to the Plant Circadian Clock -- Characterization of Flowering Time Mutants -- Rapid Detection of Hormonal Involvement in Light Responses -- Phytochrome and Light Signaling in Marchantia -- Analysis of Physcomitrella Phytochro…