The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of "Methods in Enzymology" along with Part C (volume 413) on Amylo…
This volume includes a core of methodologies to attack the unique experimental problems presented by protein misassembly. Emphasis is on human biology applications, the area in which there is the most interest, in which most of the work has already been done, and in which there …
The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part C (volume 413) on Amyloid…
Characterization of in vivo protein deposition Characterization of in vitro protein deposition Aggregate and precursor protein structure Cellular and organismic consequences of protein deposition.
This book discusses a broad range of basic and advanced topics in the field of protein structure, function, folding, flexibility, and dynamics. Starting with a basic introduction to protein purification, estimation, storage, and its effect on the protein structure, function, and…
This book discusses a broad range of basic and advanced topics in the field of protein structure, function, folding, flexibility, and dynamics. Starting with a basic introduction to protein purification, estimation, storage, and its effect on the protein structure, function, and…
This research is concerned with the measurement and characterization of the energy landscape of protein molecules, using single-molecule fluorescence methodology. The study focused on relatively small 62 amino-acid long IgG binding domain of protein L (PL), having simple two-sta…
Protein folding involves the ordering of a complex set of conformations of a protein in the unfolded state into the unique, native state. A current view of how this is accomplished is by hierarchical assembly of smaller folding regions within proteins. By characterizing subdomai…
The dynamic and thermodynamic properties of proteins are studied using mathematical models and computer simulations. These models are then tested by comparison with experimental results. First, a method based upon traditional statistical mechanics is developed to study the entro…
