Chromatin is the complex of DNA, histones and non-histone proteins that make up chromosomes and the region of dispersed chromosomes during the interphases and S phase of the cell cycle. It is found inside cell nuclei in eukaryotic cells. There are many important proteins in the …
"141" Result
Sort By:
Porcine somatic cell nuclear transfer (SCNT) offers new opportunities for fundamental science, medicine and agriculture. Ten countries or regions have developed the technology of porcine cloning and at least 42 groups have succeeded in producing piglets. Although successful, the…
For the most part, diagnostic clinical microbiology still relies on 19th century ideas and techniques, particularly microscopy and laboratory culture. In this thesis I investigate the utility of a new approach, whole-genome sequencing (WGS), to tackle current issues in infectiou…
The role of the E4 protein in the human papillomavirus (HPV) life cycle is an enigma even though it has varied effects on cell behaviour and organisation in overexpression studies. Full-length E4 proteins are derived from E1^E4 spliced RNA transcripts and E1^E4 proteins from div…
Two major reasons for the failure of central nervous system axon regeneration are (i) lack of neurotrophic factors available to CNS neurones and (ii) the presence of molecules that inhibit the growth of axons. In this study a gene therapy approach using adeno-associated virus 8 …
Antigenic variation processes play a central role in parasite invasion and chronic infectious disease, and are likely to respond to host immune mechanisms and epidemiological characteristics. Whether changes in antigenic variation strategies lead to net positive or negative effe…
Eukaryotic cells respond to extracellular stimuli by transmitting intracellular instructions via signalling pathways to coordinate appropriate responses. Mitogen-activated protein kinase (MAPK) pathways are often used to transmit these instructions to regulate gene expression, w…
Advances in DNA sequencing technologies, particularly the advent of next generation sequencing (NGS) platforms, have revolutionised the field of metagenomics and allowed great progress to be made in the way that microbial communities are analysed. However, the wealth of data now…
Ub has an essential role within the DNA double strand break (DSB) response which is well documented. However the role of ubiquitin (Ub) in the regulation of replication is an emerging area of research. This thesis investigates how two deubiquitinating enzymes (DUBs), POH1 and US…
In commercial barley cultivars meiotic crossover (CO) distribution is skewed to the distal regions of the paired chromosomes. This restricts recombination to these regions thereby reducing the potential genetic variation that can be exploited in plant breeding programs. The aim …
Striatal medium spiny neurons (MSNs) are the main output from the striatum, a subcortical part of the forebrain, which is the main input of the basal ganglia (BG) system. 96% of the striatum is composed of MSNs. Huntington’s disease (HD) is caused by a progressive loss of MSNs i…
Campylobacter jejuni is a leading cause of human bacterial enteritis worldwide. Consumption of contaminated poultry meat is considered a major source of infection. The use of virulent bacteriophages as a form of biocontrol to specifically reduce this pathogen in poultry (phage t…
Epilepsy effects up to 1% of the population, and up to 30% of people with epilepsy are pharmacoresistant—they continue to experience seizures despite treatment with maximal doses of multiple antiepileptic drugs. The causes of drug resistance in epilepsy remain poorly understood.…
Neutrophils are the major cellular constituent of blood leukocytes and play a central role in the inflammatory response, expressing an array of destructive molecules and antimicrobial processes that characterise the cells as front-line defenders of the innate immune system, thus…
The aim of this study was to investigate the genetic basis of selectivity in invasion of the red blood cells by the human malaria parasite Plasmodium falciparum. Multiple invasions of a single host red blood cell by more than one merozoite, which can be described or assessed in …
Cancer is a result of multiple genetic and epigenetic alterations that are acquired during tumour formation and progression. The development of cost-effective high-throughput technologies has positively contributed to the field of cancer research to identify cancerassociated gen…
Feedback node genes (FNGs) are essential for negative feedback control of inflammatory responses. By definition, their expression is controlled by both pro- and anti-inflammatory stimuli, often in a cooperative manner. This thesis investigates three FNGs, namely Dual specificity…
Ageing is a major cause of diseases in modern society and leads to many age-related diseases. In addition, rising obesity in society increases the likelihood of various diseases. The development of genetic sequencing technologies provides a novel platform to unravel the complexi…
Parasite life history theory predicts that lifetime reproductive success evolves through differential allocation of energy to life history traits constrained by trade-offs. These life history traits govern the characteristics of parasites such as their virulence, transmission an…