Prior to the work described in this thesis, a great deal of the oxytetracycline cluster (otc) had been sequenced and the putative functions of deduced gene products assigned, based on similarity to known gene products in the databases. The 3.0 kb KpnI23-MluI26b fragment located …
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The Malpighian tubule of Drosophila melanogaster is a valuable epithelial model for developmental, physiological and genetic studies. A set of 700 P{GAL4} enhancer-trap lines of D. melanogaster was screened for patterned -galactosidase reporter gene expression in the Malpighian …
A PCR strategy showed that a number of total mtDNA molecules was significantly decreased (~30%) in the striatum (no reduction in the cortex and cerebellum) of 24-month old HD mice, but not a 15 months of age, when compared to wild-type mice, suggesting mtDNA depletion is a progr…
Raf Kinase Inhibitor Protein (RKIP) was originally described as an inhibitor of the Ras-Raf-MEK-ERK pathway, exerting its action by the physical inhibition of the interaction of Raf with MEK. It has subsequently been shown to play important roles in a number of other signalling …
IRF-1 (Interferon Regulatory Factor 1) is a transcription factor first identified as a regulator of Interferon expression. Two decades after its discovery, IRF-1 has been shown to be involved in numerous other pathways including apoptosis, cell cycle regulation, DNA damage/repai…
Meiotic recombination in eukaryotes requires two orthologues of the E. coli RecA proteins, Rad51 and Dmc1. Both genes play an important role in the binding of single strand DNA, homology search, strand invasion and strand exchange resulting in Holliday junctions which are resolv…
Modelling and reconstruction of genetic regulatory networks has developed in a wide field of study in the past few decades, with the application of ever sophisticated techniques. This thesis looks at how models for genetic networks have been developed from simple Boolean represe…
Black widow spider venom (BWSV) contains high molecular weight proteins called latrotoxins (LTX) that induce catastrophic neurotransmitter release from nerve terminals, and one toxin, α-latrotoxin, is known to bind with high affinity to three neural proteins in mammals, includin…
The aim of this project was to test hypotheses relating to nucleosome positioning in Arabidopsis to provide a basis for better understanding of epigenetic transcriptional regulation in plants. Prior to this study, virtually no information existed regarding nucleosome positioning…
Rapid advancements in experimental techniques have benefited molecular biology in many ways. The experiments once considered impossible due to the lack of resources can now be performed with relative ease in an acceptable time-span; monitoring simultaneous expressions of thousan…
This research aimed to investigate the molecular regulation of odontoblast behaviour during primary, secondary and tertiary (reactionary and reparative) dentinogenesis. Using gene microarray analysis and sq-RT-PCR, evidence is presented that the changes in secretory activity of …
In this thesis I describe my investigations of applying machine learning methods to high throughput experimental and predicted biological data. The importance of such analysis as a means of making inferences about biological functions is widely acknowledged in the bioinformatics…
Nucleotide excision repair (NER) is a versatile pathway of DNA repair that deals with a variety of DNA lesions, such as UV-induced DNA damage and interstrand crosslinks. In bacteria, the UvrABC system carries out NER. In human cells, XPF and XPG are two structure-specific endonu…
The PDE 11 family of dual specificity phosphodiesterases was first identified in 2000, and has not been well characterised, although mutations in the gene have been linked to multiple disorders, including major depressive disorder, and cancer. DmPDE11 is a dual specificity phosp…
This thesis is concerned with speciation and population genetics in the plant genus Silene (section Elisanthe). The introductory chapter is a literature review covering characteristics of the species studied, and the current literature on their evolutionary dynamics and populati…
Histone modifications have been closely associated with changing levels of gene expression, but their role in determining, or possibly predicting, patterns of expression is uncertain. Here, the link between histone modifications and Hoxb gene expression in mouse embryonic stem (…
Functional genomics technologies, in which thousands of mRNAs, proteins, or metabolites can be measured in single experiments, have contributed to reshape biological investigations. One of the most important issues in the analysis of the generated large datasets is the selection…
Clusterin (CLU) is a multifunctional glycoprotein widely expressed as two isoforms. One isoform, sCLU is secreted, cytoplasmic and anti-apoptotic, the other, nCLU, is nuclear and pro-apoptotic. Seven genes, DKK3, TIMP1, CADM1, AKAP12, KLF4, RNASET2 and CLU were identified to be …