Thesiss

The Old Yellow Enzyme (OYE) family of enzymes has been shown to reduce industrially important chemicals and has been used to study quantum tunnelling during enzymatic hydrogen transfer. Though extensively studied, only mesophilic homologues have been studied within the enzyme fa…

Terpenes are one of the three most common metabolites in nature, derived from isoprene units to form the carbon skeleton building block of various terpene classes. Terpenes which are chemically modified, such as by isomerisation or oxidation, are also called terpenoids and are i…

Experiments facilitating the engineering of the PETNR active site to accommodate a range of non natural enone substrates with substituents localised on the α and β carbons of the unsaturated bond are described. In order to facilitate the high throughput purification of PETNR lib…

Over the course of evolution, Nature has crafted innumerous enzymatic machines, capable of great feats of chemistry, across nearly all walks of life. These biocatalysts can construct complex molecular scaffolds, demonstrated by the abundance of intricate natural products isolate…