Human Embryonic Stem Cell Protocols
The potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume collects some of the most interesting and useful protocols that have emerged in the area over the last several years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists o…
Read More ↓Title Statements
- Full title :Human Embryonic Stem Cell Protocols / edited by Kursad Turksen.
Summary
The potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume collects some of the most interesting and useful protocols that have emerged in the area over the last several years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Human Embryonic Stem Cell Protocols, Third Edition serves as a valuable resource to all those interested in exploring stem cell biology questions in a research setting.
Read More ↓Edition Statements
- Publication Date:2016.
- Edition:3rd ed. 2016
Publication Statements
- Publisher(s):Humana Press, Imprint, Springer New York
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2016.
Notes
Bibliographic Level Mode of Issuance: Monograph.
The Physical Object
- Description:1 online resource (XIV, 399 pages 68 illustrations, 40 illustrations in color.)
Edition Identifiers
- ISBN:1493926683, 9781493926688, 9781493926671, 1493926675, 9781493926695, 1493926691, 9781493949984, 1493949985
- DOI:10.1007/978-1-4939-2668-8.
- oclc:on1109075850
- LC Classification:QH573-671
- Dewey Decimal:571.6
Table of Contents
Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts -- Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel -- Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix -- Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate -- Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells -- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR -- Study of Gap Junctions in Human Embryonic Stem Cells -- Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions -- Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells -- Genetic Manipulation of Human Embryonic Stem Cells -- Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System -- Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells -- Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentia…
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