Membrane proteins-- production and functional characterization

Membrane proteins-- production and functional characterization

by Shukla, Arun K.

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Title Statements

  • Full title :Membrane proteins-- production and functional characterization / edited by Arun K. Shukla.

Edition Statements

  • Publication Date:©2015
  • Edition:First edition

Publication Statements

  • Publisher(s):Academic Press
  • Place of Publication:Waltham, Massachusetts
  • Language:eng
  • Publication Date:©2015

Notes

Description based upon print version of record.

5.2. Tips

The Physical Object

  • Description:1 online resource (693 p.).

Edition Identifiers

  • ISBN:0128015217, 9780128015216
  • oclc:on1125502451
  • LC Classification:QP552.M44
  • Dewey Decimal:572.696

Table of Contents

Front Cover; Membrane Proteins-Production and Functional Characterization; Copyright; Contents; Contributors; Preface; Section I: Recombinant Expression of Membrane Proteins; Chapter 1: Engineering Escherichia coli for Functional Expression of Membrane Proteins; 1. Introduction; 1.1. Green fluorescent protein as a folding reporter; 1.2. Antibiotic resistance marker protein for selection; 2. Preparation of Erythromycin-Sensitive E. coli Strain; 3. Preparation of Expression Plasmid; 3.1. Construction of expression vector for target protein-GFP-ErmC fusion; 3.2. Expression test

3.2.1. Bacterial culture for protein expression and determination of whole-cell fluorescence3.2.2. Gel-based analysis of the expressed fusion constructs; 4. Selecting Cells for Better Expression; 5. Characterizing Evolved Strains; 5.1. Basic characterizations; 5.1.1. Plasmid copy number, DNA sequencing, and transcript levels; 5.2. Plasmid curing; 5.3. Functional assays; 6. Summary; Acknowledgments; References; Chapter 2: ACEMBLing a Multiprotein Transmembrane Complex: The Functional SecYEG-SecDF-YajC-YidC Holotranslocon Protein S...; 1. Introduction; 2. ACEMBLing the HTL Multiprotein Complex

3. Purifying the…

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Subjects

Membrane Proteins

Writers

Shukla, Arun K.

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