This study demonstrates a heavy isotope coding strategy for the analysis of all types of tryptic peptides, including those that are N-terminally blocked and from the C-terminus of proteins. The method exploits differential derivatization of amine and carboxyl groups generated du…
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Protein pKas and metalloprotein reduction potentials are studied with computational methodologies based on an ab initio quantum mechanics (QM) description of the protein and a linearized Poisson-Boltzmann Equation (LPBE) description of the solvent. The practical applicability of…
Lack of affordable feeds is one of the major constraints facing small-scale fish farmers in Tanzania. This study evaluated the suitability of moringa leaf meal (MLM), cassava leaf meal (CLM) and cassava root meal (CRM) as novel ingredients in Nile tilapia, Oreochromis niloticus …
Skin, more specifically the outermost skin layer, the stratum corneum (SC), forms an extremely effective barrier, preventing both the loss of heat and water, and the ingress of micro-organisms and chemicals. Assessing the rate and extent of drug permeation into or through the sk…
Background. Millions worldwide are being exposed to arsenic through drinking water, which is associated with increased risks of skin cancer. Results from experimental research have found that dietary protein and specific amino acids may assist with arsenic methylation and conseq…
The objectives of this study were to carry out research to better understand of the formation, stability and properties of multilayer emulsions containing nano-laminated biopolymer coatings, and to utilize this information to develop food-grade delivery systems. The effect of va…
The mental health of soldiers is a growing concern as rates of depression and suicide have increased in soldiers with recently more deaths attributed to suicide than deaths due to combat in Afghanistan in 2012. Previous research has demonstrated the potential for eicosapentaenoi…
The effects of various protein ingredients (whey protein concentrate, beef gelling plasma, pork skin collagen, chicken skin collagen, and turkey skin collagen) utilized as a lean meat replacement in a model emulsion system and frankfurters were measured. The treatments consisted…
The current growth of the older population is unprecedented in U.S. history. Chronic disease and functional limitation commonly develop prior to old age, leading to prolonged physical disability and decreased well-being. The development of chronic disease and loss of independenc…
An important goal in the study of protein evolution is understanding which genetic changes that fixed in nature were selected for and why. However, understanding the functional consequence of any given mutation is a challenge because the effects of amino-acid changes are highly …
The sodium iodide symporter (NIS) transports iodide, which is necessary for thyroxine production. NIS is also a powerful transgene used to track the in vivo spread of oncolytic viruses, gene therapies, and transplanted cells via uptake of radiotracers into infected or transduced…
Brief periods of starvation are a common stressor that most animals encounter in the wild and must be able to survive in order to maximize their fitness. Starvation resistance of the adult fruit fly, Drosophila melanogaster, is thought to be primarily conferred by adult fat stor…
Pregnancy tests and related immunoassays are heavily dependent on specific and non-specific protein adsorption. These interfacial processes are affected by many factors that influence the in situ conformations of interfacially immobilised antibodies. This thesis examines a numbe…