This work presents an evaluation of the applicability of gene reporter technology to monitor Escherichia coli stress in industrial conditions with special interest in recombinant protein production. Different reporter plasmids containing promoter sequences of genes of the heatsh…
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Nowadays, there are many statistical methods available for genetic association analyses with data various designs. However, it is usually ignored in these analyses that an analytical method must be appropriate for an experimental design from which data is collected. In addition,…
\(Neisseria gonorrhoeae\) is an obligate human pathogen that can use both oxygen and nitrite as electron acceptors, but its electron transfer chain has only been partially characterised. The gonococcus encodes one azurin, Laz, and eight c-type cytochromes. The aim of this projec…
Tamoxifen is an effective and well-tolerated treatment for early disease and/or pre-menopausal patients with breast cancer (BC); although many women go on to develop resistance. Currently the five-year survival rate following Tamoxifen resistance (TR) is < 20%; hence the mechani…
Glial cell line-derived neurotrophic factor (GDNF) is a growth factor promoting survival, proliferation and differentiation of neural crest cells. Neural crest cells play an important role within mesenchymal tissues during dental pulp and calvarial bone development. GDNF also ha…
Immunotherapy for renal cell carcinoma (RCC) has yielded some clinical responses. However this approach frequently fails, possibly due to inefficient migration of T-cells to tumour tissue or immunosuppressive mechanisms within the tumour environment. To aid development of T-cell…
A study of the complete genome sequence of Streptomyces scabies 87.22, a common causative agent of scab disease of tubers including potato (Solanum tuberosum), is described. This work includes annotation of the genome and in-depth description of gene clusters likely to encode bi…
Mediation of biological functions occurs via tightly regulated signal transduction pathways. These complex cascades often employ crosstalk with other signalling pathways to exert strict control to allow for correct cellular responses. The cyclic AMP signalling pathway is involve…
Insects are ectotherms and have limited ability to regulate body temperature above or below ambient and are consequently greatly affected by temperature. The aphid \(Myzus\) \(persicae\) has an extensive distribution throughout Europe from Scandinavia to Southern Spain, represen…
Siderophores are small, high-affinity ferric iron chelators released by many microorganisms and some plants to solubilize iron. They are of great interest due to their clinical use to treat iron overload in humans, and also in relation to the development of novel antibiotics tha…
Two-component signalling systems (TCSs) are found in most prokaryotic genomes. They typically comprise of two proteins, a histidine (or sensor) kinase (HK) and an associated response regulator (RR), containing transmitter and receiver domains respectively, which interact to achi…
The aim of the project was to characterise known wnt signalling molecules during the early pronephros patterning using novel and effective bioassays in Xenopus embryos. Anterior somites have been shown previously to have unique biological activity to induce pronephros formation …
Mycobacterium tuberculosis, the causative agent of the infectious disease tuberculosis, has a distinct lipid-rich cell wall. Several anti-TB drugs target cell wall biosynthetic pathways. A good understanding of its biosynthesis will provide helpful clues for the development of n…
The bacterial twin-arginine translocase (Tat) pathway is able to export large folded proteins across the cytoplasmic membrane. It derives its name from the almost invariant twin-arginine motif present in the signal peptide of Tat substrates. Components of the Tat pathway have be…
\(Nesidiocoris\) \(tenuis\) Reuter (Hemiptera: Miridae) and \(Lysiphlebus\) \(testaceipes\) (Cresson) (Hymenoptera: Braconidae, Aphidiinae) are candidate biological control agents known to play an important role in the management of agricultural and horticultural pests in southe…
In Mycobacterium tuberculosis there are 2 distinct groEL homologues which encode the chaperonins Cpn60.1 and Cpn60.2, with the latter predicted to be the main house-keeping chaperonin. Phylogenetic analysis has revealed that the genes for the duplicated chaperonins diverged a lo…
Ageing, immunity and stress tolerance are inherent characteristics of all organisms. In animals, these traits are regulated, at least in part, by forkhead transcription factors in response to upstream signals from the Insulin/Insulin–like growth factor signalling (IIS) pathway. …
The Leukocyte Immunoglobulin-Like Receptors (LILRs) are a family of immunomodulatory transmembrane receptors with both activatory and inhibitory forms, and are primarily expressed on myelomonocytic cells. Group I LILRs include the well-characterised members LILRB1 and LILRB2, wh…
Transhydrogenase is an enzyme located in the cytoplasmic membrane of bacteria or the inner membrane of animal mitochondria. Using the energy of the proton electrochemical gradient (Δp), transhydrogenase translocates protons across the membrane whilst undergoing its redox reactio…