Although the use of hemoglobin-containing food additives is increasing, there is virtually no method available for monitoring their presence in food and dietary products. We have previously developed a competitive ELISA (cELISA) for the detection of bovine blood in food using a …
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Burkholderia DNT dioxygenase in this study (from recombinant E. coli strain PFJS39) is probably a multicomponent enzyme system that oxidizes 2,4-dinitrotoluene (DNT) to 4-methyl-5-nitrocatechol (MNC). DNT dioxygenase was purified by a four-step procedure that utilized consecutiv…
The HbS gene had a limited number of origins during history, and these can be defined by the haplotype (a set of DNA polymorphisms inherited together) of the associated β-globin gene. Five major haplotypes have been identified, and associated with different ethnic groups. These …
Most proteins associate into multimeric complexes with specific architectures, which often have functional properties like cooperative ligand binding or allosteric regulation. No detailed knowledge is available about how any multimer and its functions arose during historical evo…
Blood is a critically important human tissue, and for over a century, blood transfusions have saved the lives of countless patients. However, blood is often in short supply, and the cost of screening donated blood for immune compatibility and an ever-increasing number of blood-b…
Since the dawn of time, multicellular life has been exposed to a wide range of microbes. A share of them will not interfere with the host development or daily homeostasis, some might even contribute to it and potentially become essential, such as gut microbiota. However, a small…